The Supersensitive Bisphenol A (BPA) ELISA test kit easily and specifically detects BPA in environmental samples. GC/MS, a commonly method for quantitative BPA analysis, requires expensive instrumentation as well as complex and time consuming extraction process using hazardous organic solvents. With the aid of a simple solid phase extraction this ELISA test kit detects BPA in environment or in vitro at a ppt level (ng/L).

The analysis is based on a competitive reaction where enzyme-labelled standard BPA competes with free BPA in the sample for binding to a specific monoclonal antibody immobilised to the surface of the microtiter plate or tube. The amount of labelled BPA bound to the plate is determined by addition of a non-coloured substrate which is converted into a coloured product. The colour intensity is measured at 450 nm and is inversely proportional to the amount of BPA in the sample. The assay is calibrated using a standard solution of BPA supplied with the kit.

The assay is highly sensitive, simple and rapid to perform. The quantitative analysis ranges from 0.05µg/L-10 µg/L, sensitive enough to detect BPA in field speciments. The total time for measurement is only 2,5 hours.

The kit is available in microplate (96 wells) format.

Download the kit product-sheet here