The Linear Alkylbenzene Sulfonate (LAS) ELISA test kit specifically detects the surfactant LAS in water with high sensitivity with a simple procedure. In comparison, MBAS and HPLC, officially approve methods, require time-consuming operation for extraction and concentration.
The analysis is based on a competitive reaction where enzyme-labelled standard APE competes with free APE in the sample for binding to a specific monoclonal antibody immobilised to the surface of the microtiter plate or tube The amount of labelled APE bound to the plate is determined by addition of a non-coloured substrate which is converted into a coloured product. The colour intensity is measured at 450 nm and is inversely proportional to the amount of APE in the sample. The assay is calibrated using a standard solution of APE supplied with the kit.
The Linear Alkylbenzene Sulfonate (LAS) ELISA test kit is suitable for applications such as analyses of waste and river water, textiles, detergent formulas and optimization of washing processes.
The assay is highly sensitive, simple and rapid to perform. The standard curve working range is 0.00-0.5 mg/L. A simple solid phase extraction can be preformed on samples with very low concentrations of LAS.
The kit is available in a microplate (96 wells) format. download .pdf product sheet